同步硝化反硝化菌（Alcaligenes faecalis WT14）养殖污水脱氮效果研究

陈均利; 张树楠; 戴桂金; 张苗苗; 吴金水; 刘锋

文章摘要

陈均利,张树楠,戴桂金,张苗苗,吴金水,刘锋.同步硝化反硝化菌（Alcaligenes faecalis WT14）养殖污水脱氮效果研究[J].农业环境科学学报,2020,39(8):1811-1817.

同步硝化反硝化菌（Alcaligenes faecalis WT14）养殖污水脱氮效果研究

Nitrogen removal capacity of simultaneously nitrifying and denitrifying bacteria（Alcaligenes faecalis WT14） in breeding wastewater

投稿时间：2020-03-23

DOI：10.11654/jaes.2020-0325

中文关键词: 异养硝化-好氧反硝化菌同步脱氮养殖废水溶解氧

英文关键词: heterotrophic nitrifying-aerobic denitrifying bacteria simultaneous(de) nitrification breeding wastewater dissolved oxygen

基金项目:中国科学院战略性先导科技专项（XDA23020402）；国家自然科学基金青年科学基金项目（Y811461）；国家自然科学基金项目（41807056，41771302）

作者	单位	E-mail
陈均利	中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙农业环境观测研究站, 长沙 410125 中国科学院大学, 北京 100049
张树楠	中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙农业环境观测研究站, 长沙 410125
戴桂金	湖南省凤凰县农业特色产业中心, 湖南凤凰 416200
张苗苗	中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙农业环境观测研究站, 长沙 410125	zhangmiao0110@163.com
吴金水	中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙农业环境观测研究站, 长沙 410125 中国科学院大学, 北京 100049
刘锋	中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙农业环境观测研究站, 长沙 410125

摘要点击次数: 1525

全文下载次数: 1376

中文摘要:

为探究溶氧（Dissolved orygen，DO）控制对异养硝化-好氧反硝化（Heterotrophic nitrification-aerobic denitrification，HN-AD）菌脱氮效力的影响，本文从绿狐尾藻人工湿地底泥基质中分离出高效HN-AD菌Alcaligenes faecalis WT14，通过室内和反应器装置试验，较系统地研究了WT14的HN-AD性能和不同DO条件对其NH₄⁺-N、NO₃^--N去除能力的影响，并建立两级DO控制固定床反应器，通过DO控制分析了菌株WT14对养殖废水的处理效果。氮平衡试验表明，菌株WT14具有高效的同步硝化-反硝化能力，92.10%的NH₄⁺-N以气态形式被去除，4.16%的NH₄⁺-N被菌株WT14同化为胞内氮，同时NH₄⁺-N的存在会促进NO₃^--N的还原。DO控制试验表明，菌株WT14的NH₄⁺-N和NO₃^--N去除能力与DO浓度显著相关，低DO条件会抑制其NH₄⁺-N去除能力，但是会促进NO₃^--N去除能力，且符合Boltzmann模型，其脱氨脱硝活性的半数DO抑制浓度分别为2.53 mg·L^-1和5.40 mg·L^-1，最大NH₄⁺-N去除率和NO₃^--N去除率分别为94.0%和98.4%。在两级好氧（DO 4.00±0.30 mg·L^-1）条件下，WT14对养殖废水的NH₄⁺-N、TN和COD的去除率分别为99.3%、90.5%和97.5%，存在NO₃^--N和NO₂^--N的积累，而在连续好氧（DO 4.00±0.30 mg·L^-1）-微氧（DO 0.50±0.10 mg·L^-1）条件下，WT14对养殖废水的NH₄⁺-N、TN和COD的去除率分别为99.3%、97.6%和98.2%，且无NO₃^--N和NO₂^--N的积累。研究表明，两级DO控制中连续好氧-微氧显著促进了同步异养硝化-好氧反硝化菌WT14对NO₃^--N和NO₂^--N的还原，且不影响NH₄⁺-N和COD的去除，提高了TN去除率。

英文摘要:

To investigate the controlling effect of dissolved oxygen(DO)on the nitrogen removal efficiency of heterotrophic nitrifying-aerobic denitrifying(HN-AD)bacteria, Alcaligenes faecalis strain WT14, an HN-AD bacterium, was newly isolated from a constructed wetland, and its heterotrophic nitrification-aerobic denitrification capacity was explored. The effects of different DO conditions on its removal capacity of NH₄⁺-N and NO₃^--N were studied, and a two-stage, DO-controlled, fixed-bed reactor was established to analyze the effect of treatment with strain WT14 on breeding wastewater by DO control. Nitrogen balance experiments revealed that strain WT14 had an efficient and simultaneous nitrification-denitrification capacity; 92.10% of NH₄⁺-N was converted into nitrogen-containing gas, 4.16% of NH₄⁺-N was assimilated into intracellular nitrogen, and the presence of NH₄⁺-N promoted the reduction in NO₃^--N. The DO control experiment showed that the NH₄⁺-N and NO₃^--N removal capacities of strain WT14 were significantly related to the DO concentration. Low DO conditions inhibited the removal of NH₄⁺-N but promoted that of NO₃^--N, which was in accordance with the Boltzmann model. The halfinhibitory concentrations of the NH₄⁺-N and NO₃^--N removal capacities were 2.53 mg·L^-1 and 5.40 mg·L^-1 of DO, respectively. The maximum NH₄⁺-N and NO₃^--N removal efficiencies of strain WT14 were 94.0% and 98.4%, respectively. Under two-stage aerobic conditions(4.00±0.30 mg·L^-1 of DO), the removal efficiencies of NH₄⁺-N, total nitrogen(TN), and chemical oxygen demand(COD)in aquacultural wastewater by strain WT14 were 99.3%, 90.5%, and 97.5%, respectively. Under continuous aerobic(4.00±0.30 mg·L^-1 of DO)and hypoxic(0.50±0.10 mg·L^-1 of DO)conditions, the removal efficiencies of NH₄⁺-N, TN, and COD for breeding wastewater were 99.3%, 97.6%, and 98.2%, respectively, without the accumulation of NO₃^--N and NO₂^--N.

HTML 查看全文查看/发表评论下载PDF阅读器