文章摘要
王晨骄,陈颖,李蕾,郭嘉航,唐光美,官会林,黄晶心.兼具苯酚降解和禾谷镰刀菌生防功能菌的活性验证[J].农业环境科学学报,2020,39(11):2548-2553.
兼具苯酚降解和禾谷镰刀菌生防功能菌的活性验证
Activity verification of a bacterium capable of facultative phenol degradation and biocontrol of Fusarium graminearum
投稿时间:2020-04-09  
DOI:10.11654/jaes.2020-0409
中文关键词: 苯酚降解菌  生物防治  土传镰刀菌病害  分离  鉴定
英文关键词: phenol degradation  biological control  soil-borne Fusarium disease  isolation  identification
基金项目:国家自然科学基金青年科学基金项目(31800443);云南省教育厅基金项目(2018JS144);云南师范大学博士启动基金项目(2017ZB021);云南省大学生创新创业训练计划项目(DC2019-098)
作者单位E-mail
王晨骄 云南师范大学高原特色中药材种植土壤质量演变退化与修复云南省野外科学观测研究站, 昆明 650500  
陈颖 云南师范大学高原特色中药材种植土壤质量演变退化与修复云南省野外科学观测研究站, 昆明 650500  
李蕾 云南师范大学高原特色中药材种植土壤质量演变退化与修复云南省野外科学观测研究站, 昆明 650500  
郭嘉航 云南师范大学高原特色中药材种植土壤质量演变退化与修复云南省野外科学观测研究站, 昆明 650500  
唐光美 云南师范大学高原特色中药材种植土壤质量演变退化与修复云南省野外科学观测研究站, 昆明 650500  
官会林 云南师范大学高原特色中药材种植土壤质量演变退化与修复云南省野外科学观测研究站, 昆明 650500  
黄晶心 云南师范大学高原特色中药材种植土壤质量演变退化与修复云南省野外科学观测研究站, 昆明 650500 huangjingxin17@163.com 
摘要点击次数: 1350
全文下载次数: 1375
中文摘要:
      为同时解决农业用地存在的苯酚污染和土传禾谷镰刀菌病害问题,通过微生物学方法筛选高效降解苯酚的微生物,进一步从中复筛出对禾谷镰刀菌病害具有抑制作用的“多功能菌”,并通过温室试验验证该种多功能菌在降解土壤苯酚污染和防治土传镰刀菌病害的效果。本研究共分离得到的苯酚降解菌中有3株对禾谷镰刀菌具有良好的抑制效果,其中菌株PBC01在70 h内对500 mg·L-1的苯酚降解率为99.57%,该菌株对玉米禾谷镰刀菌的抑制率为79.38%,经16s rRNA基因测序鉴定该种微生物为Rhodococcus sp.。温室培养试验结果显示该菌株能显著降低土壤中的苯酚含量,40 d内将土壤中苯酚降低84.20%。同时,菌株PBC01也可减缓禾谷镰刀菌对玉米株高、叶绿素含量、生物量的抑制作用。
英文摘要:
      To simultaneously solve the problems of soil phenol pollution and soil-borne Fusarium graminearum disease in agricultural plantations, bacteria with the ability to degrade phenol efficiently were isolated. "Multifunctional bacteria" that could inhibit Fusarium graminearum were screened, and their effects on degrading phenol pollution in soil and controlling F. graminearum were verified in a greenhouse experiment. In the study, three isolated bacteria showed phenol degrading functions as well as the ability to inhibit F. graminearum. One of them, PCB01, could degrade phenol by 99.57% in a 500 mg·L-1 solution within 70 h, and exhibited an inhibition rate of 79.38% for F. graminearum. This strain was identified as Rhodococcus zopfii sp. by 16s rRNA gene sequencing. The results of the greenhouse experiment showed that the PCB01 strain degraded phenol in soil significantly (by 84.20%)within 40 d. The PCB01 strain relieved the inhibition of F. graminearum on maize and reduced the effect of F. graminearum on plant height, chlorophyll content, and single biomass. The "multifunctional bacterium" isolated in our study is potentially useful for mitigating soil phenol pollution and F. graminearum disease in maize simultaneously, and thus, it may serve as a good solution for the current high incidence of soil phenol pollution and crop disease.
HTML    查看全文   查看/发表评论  下载PDF阅读器