| 杨亦文,陈颖熙,蔡影峰,邢斯程,吴芮庭,陈凝雪,廖新俤.猪场废水处理系统出水及周边河流中噬菌体携带抗性基因的污染特征[J].农业环境科学学报,2020,39(11):2631-2639. |
| 猪场废水处理系统出水及周边河流中噬菌体携带抗性基因的污染特征 |
| Pollution characteristics of bacteriophage resistance genes in pig farm wastewater treatment system effluent and surrounding rivers |
| 投稿时间：2020-06-11 |
| DOI：10.11654/jaes.2020-0659 |
| 中文关键词: 噬菌体 抗性基因 废水处理系统 猪场 河流 |
| 英文关键词: bacteriophage antibiotic resistance gene wastewater treatment system pig farm river |
| 基金项目:广东省科技创新战略专项资金（重点领域研发计划）（2018B020205003） |
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| 摘要点击次数: 1819 |
| 全文下载次数: 1929 |
| 中文摘要: |
| 噬菌体是抗生素抗性基因水平转移的重要载体，其携带的抗性基因受到广泛关注。本研究选择5座典型猪场废水处理系统为研究对象，采集其出水样、排入河流的上游和下游水样，提取其噬菌体DNA，利用实时荧光定量PCR技术检测四环素类抗性基因tetG、tetX和tetW、磺胺类抗性基因sul1和sul2、大环内酯类抗性基因ermA和ermB、β-内酰胺类抗性基因blaTEM、氯霉素类抗性基因cmlA，以及整合子基因intl1和intl2的含量。结果表明，猪场废水处理系统出水中噬菌体携带抗性基因的检出率低于河流样品的检出率，其中只有在1/5的系统出水样品中检测到cmlA，2/5的系统出水样品中检测到sul1，而所有河流样品都检测到cmlA和sul1；sul2的平均绝对丰度（4.09±0.16）显著高于其他抗性基因的丰度（P<0.05），其他抗性基因丰度由高到低依次为tetX、tetG、sul1、tetW、blaTEM、ermB、cmlA、ermA，并且cmlA的丰度分别与blaTEM和sul1之间存在显著强正相关（P<0.05），intl1分别与blaTEM、cmlA和sul1之间呈显著强正相关（P<0.05）；只有cmlA和sul1的丰度在各猪场之间没有显著差异，其他噬菌体携带抗性基因的丰度在不同猪场间存在较大的差异；系统出水（2.01±0.21）中总抗性基因的丰度显著低于河流上游（3.03±0.13）和下游（2.88±0.16）中的丰度（P<0.05）。本研究表明，典型猪场废水处理系统出水中含有高丰度的噬菌体携带抗性基因，但未对周边河流造成显著影响。 |
| 英文摘要: |
| In this study, five typical pig farm wastewater treatment systems were selected as the research objects. The effluent, upstream water, and downstream water samples were collected, and the bacteriophage DNA was extracted. Real-time PCR technology was used to detect the abundances of tetracycline resistance genes (tetG, tetX, and tetW), sulfonamide resistance genes (sul1 and sul2), macrolide resistance genes (ermA and ermB), β-lactam resistance genes (blaTEM), chloramphenicol resistance genes (cmlA), and integrin genes (intl1 and intl2). The results showed that the detection rate of phage resistance genes in the effluent was lower than that in the river samples. Among them, cmlA was only detected in 1/5 of the effluent samples, and sul1 was only detected in 2/5 of the effluent samples. However, cmlA and sul1 were detected in all the river samples. The average absolute abundance of sul2 was 4.09 ±0.16, which was significantly higher than that of the other antibiotic resistance genes (P<0.05). There was a significant strong positive correlation between the abundance of cmlA and the abundances of blaTEM and sul1, respectively (P<0.05). Furthermore, there was a strong positive correlation between the abundance of intl1 and the abundances of cmlA, blaTEM, and sul1, respectively (P<0.05). There was no significant difference between the abundances of cmlA and sul1 in different pig farm samples. However, there was a clear difference among the abundances of other genes in different pig farm samples. The abundance of total resistance genes in the effluent was significantly lower than that in the upstream samples and downstream samples (P<0.05). This study shows that there is a high abundance of bacteriophage resistance genes in the effluent of typical pig farm wastewater treatment systems, but it does not have a significant impact on the surrounding rivers. |
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