| 邹威,金彩霞,魏闪,Ramasamy Rajesh Kumar,周启星.华北地区不同规模畜禽养殖场粪便中抗生素抗性基因污染特征[J].农业环境科学学报,2020,39(11):2640-2652. |
| 华北地区不同规模畜禽养殖场粪便中抗生素抗性基因污染特征 |
| Occurrence of antibiotic resistance genes in livestock farms of different scales in North China |
| 投稿时间：2020-04-10 |
| DOI：10.11654/jaes.2020-0403 |
| 中文关键词: 抗生素抗性基因 畜禽养殖业 有机质 碳氮比 |
| 英文关键词: antibiotic resistance genes livestock and poultry industries organic matter carbon to nitrogen ratio |
| 基金项目:教育部新世纪优秀人才支持计划（NCET-11-0254）；河南省科技攻关项目（202102310597）；河南师范大学博士科研启动基金（5101219170133） |
| 作者 | 单位 | E-mail | | 邹威 | 河南师范大学环境学院, 黄淮水环境污染与防治教育部重点实验室, 河南省环境污染控制重点实验室, 水处理关键技术国际联合实验室, 河南 新乡 453007
南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室/天津市城市生态环境修复与污染防治重点实验室, 天津 300350 | | | 金彩霞 | 河南师范大学环境学院, 黄淮水环境污染与防治教育部重点实验室, 河南省环境污染控制重点实验室, 水处理关键技术国际联合实验室, 河南 新乡 453007 | | | 魏闪 | 河南师范大学环境学院, 黄淮水环境污染与防治教育部重点实验室, 河南省环境污染控制重点实验室, 水处理关键技术国际联合实验室, 河南 新乡 453007 | | | Ramasamy Rajesh Kumar | 南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室/天津市城市生态环境修复与污染防治重点实验室, 天津 300350 | | | 周启星 | 南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室/天津市城市生态环境修复与污染防治重点实验室, 天津 300350 | zhouqx@nankai.edu.cn |
|
| 摘要点击次数: 2039 |
| 全文下载次数: 1928 |
| 中文摘要: |
| 为调查华北地区畜禽养殖环境抗生素抗性基因（ARGs）污染特征及影响因素，采集河北省和天津市不同规模的养猪场和养鸡场新鲜粪便样品，分析粪便中ARGs污染水平及有机质与ARGs含量的相关性。实时荧光定量PCR结果显示，不同种类畜禽养殖场粪便ARGs的相对丰度有显著区别，鸡粪中sul和erm基因的相对丰度高于猪粪，而编码核糖体保护蛋白的tet基因（4.24×10-3~5.85×10-1）在猪粪和鸡粪中的相对丰度无明显差异，均显著高于sul（1.07×10-4~2.26×10-1）、erm（6.36×10-4~2.62×10-1）以及编码外派泵蛋白和酶抑制剂的tet基因（1.24×10-4~5.41×10-2）。不同规模的养猪场粪便ARGs污染水平趋势为：中型 > 大型 > 小型，而不同规模养鸡场粪便中ARGs相对丰度无显著性差异（P=0.551）；此外，正交偏最小二乘判别（OPLS-DA）与典型相关分析（CCA）结果显示，畜禽粪便中编码核糖体保护蛋白的tet基因（tetM、tetO和tetW）相对丰度与有机碳（OC）和有机氮（ON）含量高度相关（VIP> 1），sul基因则与OC/ON明显相关。综上，粪便中有机质和生物可利用碳氮比是影响畜禽养殖业ARGs污染水平的重要因素。 |
| 英文摘要: |
| To investigate the contamination profile and influence factors of antibiotic resistance genes (ARGs)in livestock and poultry industries in Northern China, the animal feces were collected from swine and chicken farms of different sizes in Hebei Province and Tianjin. In the experiment, the ARG abundances and their relationship with organic matter in manures was determined. The results of realtime fluorescent quantitative polymerase chain reaction indicated that the relative abundances of sul and erm in chicken feces were significantly higher than those in swine feces. In contrast, the levels of tet encoding ribosome protection protein (RPP)resistance in swine and chicken feces were similar (4.24×10-3~5.85×10-1), but higher than those of sul (1.07×10-4~2.26×10-1), erm (6.36×10-4~2.62×10-1), and tet encoding efflux pump proteins and enzyme-inhibitor complex resistance (1.24×10-4~5.41×10-2). The relative abundances of ARG in swine feedlots of different sizes were ranked as:middle > large > small, whereas no significant difference in ARG level was found between middle-and small-sized chicken farms (P=0.551). Further, the orthogonal partial least squares discriminant and canonical correspondence analyses revealed that tet encoding RPP resistances (tetM, tetO, and tetW)were significantly correlated with organic carbon (OC)and organic nitrogen (ON)contents (variable important in projection > 1), while the OC/ON ratio was relevant to the prevalence of the sul Ⅰ and sul Ⅱ genes. The above findings indicate that bioavailable organic matter in animal manures may be a key factor in determining ARG pollution in the livestock and poultry industries. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
