| 原海燕,刘清泉,张永侠,符佳豪,王银杰,孙玉明,佟海英.纳米硫对铅胁迫下油菜幼苗生长和铅积累的影响[J].农业环境科学学报,2021,40(3):517-524. |
| 纳米硫对铅胁迫下油菜幼苗生长和铅积累的影响 |
| Effects of sulfur nanoparticles on growth and Pb accumulation in Brassica napus L. seedlings under Pb stress |
| 投稿时间：2020-07-13 |
| DOI：10.11654/jaes.2020-0798 |
| 中文关键词: 纳米硫 油菜 铅胁迫 积累 抗氧化酶 |
| 英文关键词: sulfur nanoparticles Brassica napus L. Pb stress accumulation antioxidant enzymes |
| 基金项目:江苏省重点研发计划（社会发展）项目（BE2018715）；国家自然科学基金青年科学基金项目（41907135）；江苏省自然科学基金青年科学基金项目（BK20180317）；江苏省属公益类科研院所自主科研课题（BM2018021-6） |
|
| 摘要点击次数: 1182 |
| 全文下载次数: 1026 |
| 中文摘要: |
| 为探究纳米硫（SNPs）对植物生长和重金属积累的影响，以油菜为研究材料，通过在培养基中添加SNPs和铅（Pb），研究了Pb胁迫下SNPs对油菜幼苗生长、生理以及Pb积累的影响。结果表明，1.2 mmol·L-1 Pb对油菜种子发芽率没有影响，但显著抑制了幼苗的生长，油菜鲜质量较对照（Pb0）下降了38.9%。同时，Pb胁迫导致膜脂过氧化严重，油菜地上部和根系膜脂过氧化产物丙二醛（MDA）含量分别为对照的3.7倍和2.5倍，且地上部和根系超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）等抗氧化酶活性响应Pb逆境胁迫均有所上升。而Pb胁迫下添加300 mg·L-1 SNPs与单一Pb胁迫相比，油菜地上部和根系Pb含量仅为Pb单一处理的5.7%和29.5%，干质量分别较Pb单一处理增加70.7%和26.1%，同时MDA含量和抗氧化酶活性均低于Pb单一胁迫下水平。另外，Pb胁迫下添加与SNPs同浓度大颗粒硫（SBPs）和硫酸盐的研究结果也显示，SNPs对油菜吸收Pb的抑制作用和毒害缓解作用显著优于SBPs和硫酸盐。研究表明，SNPs可极大地阻控油菜对Pb的吸收并显著缓解Pb对油菜的毒害。 |
| 英文摘要: |
| To explore the effects of sulfur nanoparticles(SNPs) on the growth and heavy metal accumulation in plants, their effects on the growth, physiology and Pb accumulation in Brassica napus L. seedlings were studied using Murashige and Skoog(MS) media culture with additions of Pb and SNPs. The results showed that 1.2 mmol·L-1 Pb had no obvious effect on seed germination although it did significantly prohibit the growth of the Brassica seedlings and decrease the fresh weight thereof by 38.9% compared with that of the control(Pb0). Pb stress also resulted in serious lipid peroxidation, and the malondialdehyde(MDA) contents in the shoots and roots of B. napus were independently, 3.7 and 2.5 times that of the control, respectively. Moreover, the activities of antioxidant enzymes including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX) were all induced and increased by Pb stress. However, in contrast with the single Pb stress treatment, the Pb contents in shoots and roots exposed to the combined Pb+SNPs treatment were only 5.7% and 29.5% those of Pb stress treatment, respectively. Furthermore, the dry weights of shoots and roots increased by 70.7% and 26.1% over those in the single Pb stress treatment, and the MDA contents and antioxidant enzymes activities thereof were all lower than the levels in the latter. In addition, SNPs had a more significant preference for inhibiting Pb uptake and alleviating Pb toxicity compared to sulfur bulk particles(SBPs) and sulfate. Therefore, SNPs plays an incredibly important role in prohibiting Pb absorption and alleviating Pb toxicity in B. napus. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
