| 王天廓,温玉娟,杨悦锁,路莹,张茜,曹楠,孙东.一株草甘膦高效降解菌的筛选和表征研究[J].农业环境科学学报,2021,40(3):591-599. |
| 一株草甘膦高效降解菌的筛选和表征研究 |
| Isolation and characterization of high-efficiency glyphosate-degrading bacteria |
| 投稿时间：2020-12-06 |
| DOI：10.11654/jaes.2020-1404 |
| 中文关键词: 草甘膦 生物降解 剑菌属(Ensifer sp.) 降解菌 Haldane模型 |
| 英文关键词: glyphosate bioremediation Ensifer sp. degrading bacteria Haldane's model |
| 基金项目:国家重点研发计划项目（2018YFC1800904）；辽宁省重点研发新项目（2020JH2-10300083）；国家自然科学基金项目（41703125）；辽宁兴辽英才计划项目（XLYC1807259）；高等学校学科创新引智计划资助项目（B16020） |
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| 中文摘要: |
| 为研究利用细菌微生物降解草甘膦农药污染，从沈阳某地区农田土壤中分离得到一株草甘膦高效降解菌Ensifer sp.BRY。基于16S rDNA检测，BRY被鉴定为剑菌属（Ensifer sp.）。BRY能在以草甘膦（最高浓度400 mg·L-1）为唯一碳源的无机盐培养基中生长，在50 h对300 mg·L-1草甘膦的降解率可达到69.60%。在30℃、pH 6.0、10%初始接种量时，菌株BRY在50 h内的草甘膦（100 mg·L-1）降解率达到91.93%，当相同条件下调节初始接种量为20%时，菌株BRY的草甘膦降解率升高。当培养体系加入其他碳源（葡萄糖、蔗糖）时，草甘膦降解率降低。菌株BRY对不同浓度草甘膦的降解过程符合Haldane方程，其最大比生长速率μmax为1.68 h-1，半饱和常数Ks为167.80 mg·L-1，抑制常数Ksi为50.55 mg·L-1，Ksi/Ks为0.30。研究表明，菌株BRY对草甘膦具有较高的耐受能力和降解能力，通过优化培养条件可以提高降解效率，在用于草甘膦污染环境的生物修复过程中，菌株BRY具有独特潜力。 |
| 英文摘要: |
| Pollution from pesticides is a primary eco-health concern, in which glyphosate eco-treatment is a particularly controversial topic. In this study, an efficient glyphosate-degradation bacteria Ensifer sp. BRY was isolated and screened from farmland soil in Shenyang, China, where various pesticides have been applied for extended periods. Strains were identified by 16s rDNA sequence homology analysis. The optimal growth and glyphosate removal characteristics of strain BRY were explored by an environmental singlefactor experiment. Kinetic curve fitting was performed on the removal processes of different initial glyphosate mass concentrations using the Haldane equation. Results show that the strain BRY identified as Ensifer sp. BRY can grow in an inorganic salt medium with glyphosate (maximum concentration of 400 mg·L-1) as the sole carbon and energy source. The glyphosate degradation rate(300 mg·L-1) can reach 69.60% within 50 h. The glyphosate degradation rate(100 mg·L-1) of strain BRY reached 91.93% within 50 h at 30℃, pH 6.0, and a 10% initial inoculation. The glyphosate degradation rate of strain BRY increased when the initial inoculum was adjusted to 20% under the same conditions and did not change significantly when the initial inoculum was increased to 30%, indicating that the optimal initial inoculation amount for BRY to degrade glyphosate was 20%. When other carbon sources(glucose, sucrose) were added to the culture system, the glyphosate degradation rate decreased. The glyphosate degradation process of strain BRY agrees with the Haldane equation. According to Haldane's model, the kinetic parameters for the glyphosate-grown strain BRY were μmax=1.68 h-1, Ks=167.80 mg·L-1, Ksi=50.55 mg·L-1, and Ksi/Ks=0.30. Based on these results, the strain BRY shows particular potential in the bioremediation of a glyphosate-contaminated environment. |
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