| 闫雷,丁工尧,杨凤霞,阮蓉,韩秉君,支苏丽,张克强.天津市畜禽粪污处理工艺对抗生素抗性基因的去除效果[J].农业环境科学学报,2021,40(9):2021-2030. |
| 天津市畜禽粪污处理工艺对抗生素抗性基因的去除效果 |
| Pilot-scale study on effects of exogenous additives on reducing NH3 and H2S emissions from pig manure compost |
| 投稿时间：2020-11-06 |
| DOI：10.11654/jaes.2020-1280 |
| 中文关键词: 抗生素抗性基因 相对丰度 畜禽粪污 处理工艺 去除效果 |
| 英文关键词: antibiotic resistance gene relative abundance livestock and poultry manure treatment process removal efficiency |
| 基金项目:中央级公益性科研院所基本科研业务费专项（2020-jbkyywf-zsl）；国家自然科学基金项目（41807399，41807474）；农业部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室开放基金课题“四环素类抗生素的土壤环境行为及生态毒性研究” |
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| 中文摘要: |
| 为了探明养殖场粪污处理工艺对抗生素抗性基因（ARGs）的去除效果，选取天津市不同畜种且具有典型处理工艺的养殖场为研究对象，运用实时荧光定量PCR技术，对养殖场粪便和污水样品中5类13种ARGs和intI1进行检测。结果表明：目标ARGs和intI1在畜禽粪便和污水中普遍存在（10-7~10-1），猪、鸡和牛3种养殖场粪便中喹诺酮类ARGs的相对丰度最低（10-7~10-5），四环素类和磺胺类ARGs的相对丰度较高，分别为10-4~10-1和10-4~10-2，猪场污水中13种ARGs和intI1的相对丰度（10-3~10-2）普遍高于牛场（10-6~10-4）。对于畜禽污水，猪场和牛场的处理工艺可以去除大部分ARGs，尤其是四环素类ARGs，相对丰度（lg值）下降了0~2.09，此外，猪场污水处理工艺中厌氧发酵单元和贮存池单元对ARGs的去除效果较好，牛场污水处理工艺中调节池单元对ARGs的去除效果较好；对于畜禽粪便，异位发酵床、堆肥和无菌晾晒处理均能去除大部分目标ARGs。猪粪在异位发酵床处理后，10种ARGs的相对丰度下降，其lg值平均降低了1.11；牛粪和鸡粪在堆肥后，分别有8种和9种ARGs的相对丰度下降，其lg值平均降低了0.83和1.32；牛粪在灭菌晾晒处理后，5种ARGs的相对丰度下降，其lg值平均降低了1.12。磺胺类ARGs在以上处理工艺中呈现升高趋势，因此，应该对磺胺类ARGs引起的污染予以重视。 |
| 英文摘要: |
| In order to investigate the removal efficiency of antibiotic resistance gene(ARGs) from livestock and poultry manure by treatment processes, different livestock farms in Tianjin City that have typical treatment processes were selected to detect of 13 ARGs (comprising 5 types) and intI1 in feces and sewage using real-time fluorescence based quantitative PCR. The results revealed that the target ARGs and intI1 were highly prevalent in feces and sewage(10-7~10-1). Among these, the relative abundance of quinolone ARGs was lowest(10-7~10-5) in feces on the pig, chicken and cattle farms, and the levels of tetracycline and sulfonamide ARGs contamination were relatively higher, with relative abundances of 10-4~10-1 and 10-4~10-2, respectively. The relative abundances of the 13 ARGs and intI1 in pig farm sewage(10-3~10-2) were generally higher than those in cattle farm sewage(10-6~10-4). For ARGs in sewage, the treatment processes on pig and cattle farms could remove most ARGs, particularly tetracycline ARGs, with relative abundance(lgs) reduced by 0~2.09. In addition, the anaerobic fermentation and storage units used for pig farm wastewater treatment processes showed better efficiency for the removal of ARGs, whereas adjustment units were more efficient in the removal of ARGs on cattle farms. With respect to livestock and poultry feces, the ectopic fermentation bed, composting, and aseptic drying treatments could remove most of the target ARGs. Treatment of pig manure by ectopic fermentation beds could reduce the relative abundance(lgs) of 10 ARGs, with an average reduction of 1.11, whereas after the composting of cattle and chicken manure, the relative abundance(lgs) of 8 and 9 ARGs was reduced by 0.83 and 1.32, respectively. Furthermore, after sterilization and drying, the relative abundances(lgs) of 5 ARGs in cattle manure was reduced by 1.12 on average. However, it is worth noting that the levels of sulfonamide ARGs showed an increasing trend after these treatment processes, and therefore future studies should focus on the contamination caused by sulfonamide resistance genes. |
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