| 韩秉君,沈仕洲,杨凤霞,高文萱,丁永祯,张克强.不同施肥模式对洱海流域稻田土壤中耐药基因污染的影响[J].农业环境科学学报,2021,40(11):2503-2512. |
| 不同施肥模式对洱海流域稻田土壤中耐药基因污染的影响 |
| Effects of different fertilization modes on the pollution of antibiotic resistance genes in paddy soils of Erhai Lake basin |
| 投稿时间：2021-09-08 |
| DOI：10.11654/jaes.2021-1044 |
| 中文关键词: 粪肥还田 施肥模式 抗生素耐药基因 污染谱图 分布模式 |
| 英文关键词: manure application fertilization mode antibiotic resistance gene pollution spectrum distribution patterns |
| 基金项目:所级基础前沿任务资助项目（2021-jcqyrw-yfx）；国家自然科学基金项目（41807399，42077355）；中央级公益性科研院所基本科研业务费专项（Y2021PT01）；云南省重大科技专项计划项目（202102AE090011） |
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| 中文摘要: |
| 为探究不同施肥模式对洱海流域稻田土壤抗生素耐药基因（ARGs）和可移动遗传元件（MGEs）的影响，通过长期田间定位试验，采用高通量荧光定量PCR技术及实时荧光定量PCR技术相结合的方法，对不同施肥模式下稻田土壤中的144种ARGs和10种MGEs进行检测，全面解析了稻田ARGs的污染状况及空间分布规律。结果表明：不同施肥处理土壤中ARGs分布特征不同，其赋存绝对丰度水平整体呈现施用粪肥 > 施用缓控释肥 > 施用化肥 > 不施肥；施加粪肥土壤中的ARGs的赋存多样性和MGEs的种类显著高于其他处理土壤，不施肥模式则显著低于其他处理模式（P<0.05）。不同ARGs的相对丰度在稻田土壤柱中出现垂直迁移富集趋势，但其在不同土层中的富集程度存在差异。不同施肥模式稻田土壤各土层中的优势ARGs为多重耐药类（占比均值为60.3%）、氨基糖苷类（占比均值为10.5%）和四环素类（占比均值为7.5%），其水平多与MGEs丰度显著相关（P<0.05），表明MGEs促进了稻田土壤中ARGs的传播，加剧了土壤中ARGs的积累富集。研究表明，不同施肥模式对稻田土壤中ARGs和MGEs的分布规律有显著影响，不同深度土层中ARGs的富集程度各异。 |
| 英文摘要: |
| To explore the effects of different fertilization modes on antibiotic resistance genes(ARGs)and mobile genetic elements(MGEs) in paddy soils of Erhai Lake basin, 144 ARGs and 10 MGEs in paddy soil under different fertilization modes were detected using high-throughput quantitative PCR and real-time quantitative PCR to comprehensively analyze the pollution status and spatial distribution of ARGs in paddy fields. The results showed that the distribution patterns of ARGs were different in different fertilization treatments; the absolute abundance of ARGs in the soil was in the order of manure application > slow and controlled release fertilizer application > chemical fertilizer treatment > no fertilization. The occurrence and diversity of ARGs and the types of MGEs in soil with manure application were significantly higher than those in other treatments, whereas those in the non-fertilization treatment were significantly lower than those in other treatments. The relative abundance of different ARGs showed a vertical migration and enrichment trend in the paddy soil column, but their enrichment degree was different in different soil layers. The dominant ARGs in each paddy soil layer under different fertilization modes were multidrug(average proportion of 60.3%), aminoglycoside(average proportion of 10.5%), and tetracycline(average proportion of 7.5%). Their levels were significantly correlated with the abundance of MGEs(P<0.05), thereby indicating that MGEs promoted the spread of ARGs and aggravated the accumulation and enrichment of ARGs in the soil. The distribution of ARGs and MGEs in paddy soil was significantly affected by different fertilization modes, and the enrichment degree of ARGs in different soil layers was different. |
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