| 周聪,陈未,高岩,施曼,李江叶,刘丽珠,陈金林.氟磺胺草醚及其降解菌对大豆生长及生物固氮的影响[J].农业环境科学学报,2021,40(12):2660-2668. |
| 氟磺胺草醚及其降解菌对大豆生长及生物固氮的影响 |
| Effects of fomesafen and its degrading bacteria on soybean growth and biological nitrogen fixation |
| 投稿时间：2021-03-05 |
| DOI：10.11654/jaes.20211208 |
| 中文关键词: 氟磺胺草醚 大豆 根瘤菌 生物固氮 氨氧化古菌(AOA) 氨氧化细菌(AOB) |
| 英文关键词: fomesafen soybean rhizobium biological nitrogen fixation ammonia oxidizing archaea(AOA) ammonia oxidizing bacteria (AOB) |
| 基金项目:江苏高校优势学科建设工程项目；江苏省农业科技自主创新资金项目[CX（20）1010]；河北省湿地生态与保护重点实验室（筹）开放基金项目（hklk202005） |
|
| 摘要点击次数: 1244 |
| 全文下载次数: 1180 |
| 中文摘要: |
| 从生长于氟磺胺草醚污染土壤的大豆根瘤中筛选出的Sinorhizobium sp.W16菌株，能高效降解氟磺胺草醚并能缓解氟磺胺草醚的生物负效应。以大豆（苏C1008）为研究对象，采用盆栽试验，探究Sinorhizobium sp.W16对大豆生长、氮累积量、根瘤固氮酶活性及根际土壤氮循环相关微生物功能基因丰度等的影响。结果表明：氟磺胺草醚的施用量（以有效成分计）超过450 g·hm-2时显著降低了大豆生物量，抑制了大豆根瘤固氮酶活性和土壤脲酶活性，降低了土壤固氮细菌（nifH）、氨氧化古菌（AOA）、氨氧化细菌（AOB）基因丰度，限制了植物-根际系统的生物固氮及有机氮素转化；接种Sinorhizobium sp.W16降解菌显著提高土壤中氟磺胺草醚的降解率至81.97%，且显著提高了大豆根瘤干质量、根瘤固氮酶活性和土壤nifH基因丰度，增强了大豆的固氮作用，同时刺激了脲酶活性，提升土壤AOA和AOB的基因丰度，增加了土壤有效氮素的供应，从而使大豆植株氮含量提高了15.85%~24.93%。研究表明，氟磺胺草醚的施用抑制了大豆-根际系统的生物固氮作用，但接种Sinorhizobium sp.W16降解菌不仅能有效降低土壤中氟磺胺草醚的残留量、缓解氟磺胺草醚对大豆的持续药害，还增强了植物-根际系统中生物固氮能力、土壤有效氮素供应及大豆的氮素累积，对修复氟磺胺草醚污染土壤、增强大豆固氮具有较好的应用价值和市场前景。 |
| 英文摘要: |
| A fomesafen-degrading bacteria strain, Sinorhizobium sp. W16, was isolated and screened from root nodules of soybean grown in soil contaminated with fomesafen; however, whether inoculation of this strain could improve soybean growth and the biological nitrogen fixation capacity in plant-soil system remains unclear. A pot experiment was carried out to investigate the effects of Sinorhizobium sp. W16 inoculation on soybean(Su C1008) growth, nitrogen content, nodule nitrogenase activity, and nitrogen cycle-related functional genes abundance in the rhizosphere soil. The results showed that the use of fomesafen(calculated by available ingredient) over 450 g·hm-2 significantly reduced soybean biomass, inhibited the activities of nitrogen fixing enzymes in soybean nodules and urease in rhizospheresoil, and decreased the gene abundance of nitrogen-fixing bacteria(nifH), ammonia oxidizing archaea(AOA), ammonia oxidizing bacteria (AOB) in the rhizosphere-soil, and thereby limited the biological nitrogen fixation and organic nitrogen transformation in plantrhizosphere system. Inoculation of the Sinorhizobium sp. W16 strain significantly improved the degradation rate of fomesafen by up to 81.97%, and enhanced the dry weight of soybean nodules, activities of nodule nitrogenase and gene abundances of soil nifH, resulting in the increase of nitrogen fixation in soybeans-rhizosphere system. Meanwhile, the strain inoculation stimulated urease activity and enhanced the abundance of soil AOA and AOB, leading to the increase of the available nitrogen in the rhizosphere-soil. Combined, these effects of Sinorhizobium application led to the increase in nitrogen content of soybean plants by 15.85%~24.93%.The use of fomesafen significantly inhibited the nitrogen fixation in soybeans-rhizosphere system; however inoculation of the Sinorhizobium sp. W16 strain could not only degrade the fomesafen in soil effectively, alleviating the totoxicity of fomesafen to soybeans, but could also enhance the biological nitrogen fixation and available nitrogen in the soybeans-rhizosphere system, and nitrogen accumulation by soybeans, implying a good prospect for remediating fomesafen-contaminated soil and enhancing soybean nitrogen fixation. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
