| 林晓燕,曹赵云,牟仁祥,吴莉,朱智伟,陈铭学.两种基体改进剂在蔬菜样品农药残留基质效应中的补偿作用[J].农业环境科学学报,2015,34(12):2439-2448. |
| 两种基体改进剂在蔬菜样品农药残留基质效应中的补偿作用 |
| Compensation of Two Matrix Modifiers for Matrix Effect in Determination of Pesticide Residues in Vegetable Samples |
| 投稿时间：2015-06-16 |
| DOI：10.11654/jaes.2015.12.026 |
| 中文关键词: 基体改进剂 蔬菜样品 农药残留 基质效应 |
| 英文关键词: matrix modifier vegetable sample pesticide residue matrix effect |
| 基金项目:国家自然科学基金青年基金项目(31100405);浙江省科技计划项目(2013C37037);中央级公益性科研院所专项基金项目(2014RG006, 2014RG006-3) |
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| 中文摘要: |
| 建立了蔬菜中有机磷、有机氯和菊酯类农药等43种化合物的气相色谱-串联质谱检测方法,评价了分析过程中添加基体改进剂L-古洛糖酸γ-内酯(AP)和D-山梨醇(SLC)对农残分析中基质效应补偿作用的影响。结果表明,对大多数有机磷农药,尤其是甲胺磷、乙酰甲胺磷、氧乐果等基质敏感农药而言,AP具有显着的基质补偿作用,能够实现对基质敏感农药的保护和非基质敏感农药不受影响,平均回收率为82.4%～120.7%,RSD为0.7%～3.5%;加入基体改进剂AP的方法可以代替基质匹配校正方法进行有机磷农药的检测和定量。对于常规基质中的有机氯和菊酯类农药,只需添加AP就能实现对基质效应的补偿作用,大部分农药回收率能达到80%～120%,说明加入AP的方法同样可以代替基质匹配校正方法;对于复杂基质中的有机氯农药,除o,p'-滴滴涕和p,p'-滴滴涕外,基体改进剂的加入能够起到明显的补偿作用,且以AP和SLC的混合溶液的补偿效果最佳;对于菊酯类农药,这2种基体改进剂的3种组合补偿效果不尽相同,还需进一步研究。总之,所建立的基体改进剂方法结合简捷高效的前处理方法及高灵敏度的GC-MS/MS多残留检测方法,可用于蔬菜等农产品中农药多残留的分析检测。 |
| 英文摘要: |
| A detection method of gas chromatography tandem mass spectrometry for 43 pesticide residues in vegetables was established. Gamma-L-gulonic acid lactone(AP) and D-sorbitol(SLC) used as matrix modifiers for compensation effect of matrix were evaluated. For the majority of organophosphorus pesticides, especially the matrix-sensitive pesticides, such as methamidophos, acephate and omethoate, AP had significant matrix compensation, with an average recovery of 85.8%～116.7% and a relative standard deviation(RSD) of 1.7%～2.5%. For other organophosphorus pesticides, an average recovery of 82.4%～120.7% and a RSD of 0.7%～3.5% could be obtained by adding AP. For regular matrix, AP addition could realize the compensation effect of matrix effect, with recovery rates of 80%～120% for most of pesticides. For organochlorine pesticides in complex matrix, addition of matrix modifiers had obvious compensation effect, and the mixture of AP and SLC had the optimal compensation effect, excepting o, p'-DDT and p, p'-DDT; while for pyrethriods pesticides, the compensation effects of three combinations of two matrix modifiers were not consistent, needing further study. In short, the established method of high sensitive GC-MS/MS combined with matrix modifier addition plus simple and efficient pretreatments could be used for detection of pesticide residues in vegetables and other agricultural products. |
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